How can I delete the content of the folder?
A:
To delete an entire folder, you need a separate method (e.g. with Windows Explorer).
For this, you will need a portable version of Windows Explorer (in case you do not have one in your computer).
Open a portable version of Windows Explorer.
In Windows Explorer, click on the folder to be deleted.
In the menu bar, click on View > Properties.
Click on the Uncheck box next to "Hide protected operating system files".
This will reveal the actual folder contents in the folder you can then delete.
To do this in Linux:
You can use the rm command:
In Terminal:
rm -rf
This will delete the files, directories, and subdirectories within the folder.
In Linux, if you only want to delete a specific folder, you can use:
rm -r
Design and optimization of a cell-free protein synthesis-based assay for the detection of protein glycosylation defects.
The synthesis of complex glycans by most glycosyltransferases (GTs) is a multistep process requiring a combination of both donor and acceptor substrates. The flexibility of the GTs to use a variety of glycan substrates, each with distinct structures and stereochemistry, makes the assay of GT function challenging, and yet, essential. Here we describe the development of a cell-free protein synthesis-based assay for the detection of GTs, taking advantage of the modularity of protein synthesis to assemble a complex glycan structure in a single reaction tube. The resulting glycoconjugate is then purified and quantified using a facile assay employing a commercially available HRP conjugate. A GT identified as having a glycosylation defect was tested and shown to correctly assemble the glycan structure and display the correct glycosylation phenotype in vitro, whereas a control GT displaying the same domain was found to be correctly glycosylated. This novel approach provides a rapid, scalable, and sensitive method of investigating GT defects.It seems that Colorado Sen. Michael Bennet is taking his fundraising haul of $10,848.33 per signature and applying the method of the world's greatest poker player. Since taking office in January 2009, Bennet has been famous for his aggressive fundraising and has been among the most prolific fundraisers in the Senate.
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